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recombinant human recombinant tgf-b1 (cho derived)  (PeproTech)


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    Structured Review

    PeproTech recombinant human recombinant tgf-b1 (cho derived)
    Recombinant Human Recombinant Tgf B1 (Cho Derived), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+recombinant+tgf-b1+(cho+derived)/tgf+%CE%B21/pm36170816-256-77-85
    Average 90 stars, based on 1 article reviews
    recombinant human recombinant tgf-b1 (cho derived) - by Bioz Stars, 2026-11
    90/100 stars

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    Recombinant:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Derivative Assay:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    RNA Sequencing:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Microarray:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Infection:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Control:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Stable Transfection:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.

    Expressing:

    Article Title: Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-GFP Abcam #5450; RRID: AB_304897 Mouse monoclonal anti-Chromogranin A Santa Cruz Biotechnology #393941; RRID: AB_2801371 Mouse monoclonal anti-Erk1 (pT202/ pY204) + Erk2 (pT185/pY187) Abcam #50011; RRID: AB_1603684 Rabbit monoclonal anti-Cyclin D1 Life Technologies #MA5-14512; RRID: AB_10985779 Rabbit polyclonal anti-DCAMKL1 Abcam #31704; RRID: AB_873537 Rabbit polyclonal anti-Lysozyme C Dako #A0099; RRID: AB_2341230 Rabbit polyclonal anti-Mucin 2 Santa Cruz Biotechnology #15334; RRID: AB_2146667 Chemicals, Peptides, and Recombinant Proteins Advanced DMEM/F-12 Fisher Scientific #12634-028 AT9283 Selleck Chemicals #S1134 B-27 Supplement (50X), serum free Invitrogen #17504-044 Baricitinib (INCB028050) Selleck Chemicals #S2851 BDP-FL azide Lumiprobe #11430 DAPT Stemgent #04-0041 CHIR-99021 Sigma Aldrich #SML1046 Copper(II) Sulfate VWR International #470300-880 EdU (5-ethynyl-2’-deoxyuridine) Thermo Fisher #A10044 EGF Recombinant Mouse Protein Invitrogen #PMG8043 Erlotinib HCl (OSI-744) Selleck Chemicals #S1023 Flavopiridol HCl Selleck Chemicals #S2679 GlutaMAX Supplement Invitrogen #35050-061 HEPES (1M) Invitrogen #15630080 Hoechst 33342 Invitrogen #H3570 IWP-2 Selleck Chemicals #S7085 LDN193189 hydrochloride Sigma Aldrich #SML0559 N-2 Supplement (100X) Fisher Scientific #17502-048 N-Acetyl-L-cysteine Sigma Aldrich #A9165 P-7744 Palbociclib, Free Base, >99% LC Laboratories #-7744 Palbociclib (PD-0332991) HCl Selleck Chemicals #S1116 Penicillin-Streptomycin Solution Corning #30-002 PD0325901(Mirdametinib) Selleck Chemicals #S1036 Recombinant Human BMP-4 Protein R&D Systems #314-BP-010 Recombinant Human TGF-b1 (CHO derived) PeproTech #100-21C Recombinant Human Wnt-3a Protein R&D Systems #5036-WN-500 Recombinant Murine IL-4 PeproTech #214-14 Recombinant Murine R-Spondin-1 Peprotech #315-32 SB 202190 Sigma Aldrich #S7067 Sodium L-ascorbate Sigma Aldrich #A4034 Sulfo-Cyanine5 azide Lumiprobe #B3330 Vactosertib (EW-7197) Selleck Chemicals #S7530 Valproic acid sodium salt 98% Sigma Aldrich #P4543 Y-27632 2HCl Selleck Chemicals #S1049 (Continued on next page) e1 Developmental Cell 56, 356–365.e1–e9, February 8, 2021

    Article Title: TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-cyclin A2 (BF683) Cell Signaling Technology Cat#4656; RRID: AB_2071958 Rabbit monoclonal anti-cyclin D1 (92G2) Cell Signaling Technology Cat#2978; RRID: AB_2259616 Mouse monoclonal anti-cyclin D3 (DCS22) Cell Signaling Technology Cat#2936; RRID: AB_2070801 Rabbit polyclonal anti-a-tubulin Abcam Cat#ab4074; RRID: AB_2288001 Mouse monoclonal anti-KRTAP2-4 (OTI3B4) Thermo Fisher Scientific Cat#MA5-25738; RRID: AB_2724575 Mouse monoclonal anti-E-cadherin (4A2) Cell Signaling Technology Cat#314472S; RRID: AB_2728770 Rabbit monoclonal anti-vimentin [EPR3776] Abcam Cat#ab92547; RRID: AB_10562134 Chemicals, peptides, and recombinant proteins Recombinant human recombinant TGF-b1 (CHO derived) PeproTech Cat#100-21C; GenPept: P01137 Recombinant human recombinant TGF-b2 (HEK293 derived) PeproTech Cat#100-35B; GenPept: P61812 Recombinant human recombinant TGF-b3 (CHO-expressed) R&D System Cat#8420-B3-005; GenPept: P10600 Deposited data Raw and analyzed data of RNA-seq This paper GEO: GSE179223 Raw and analyzed data of cDNA microarray This paper GEO: GSE183524 Raw data of single cell RNA-seq (SAS cells treated with or without TGF-b) This paper DRA: DRA013187 Raw data of single cell RNA-seq (SAS cells infected with control lentivirus, KRTAP2-3- or ENC1-encoding lentivirus) This paper DRA: DRA014478 Experimental models: Cell lines Human, female: SAS (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1974; RRID: CVCL_1675 Human, male: HSC-4 (oral squamous cell carcinoma) RIKEN BioResource Center Cell Bank RCB1902; RRID: CVCL_1289 Human, female: SAS-Fucci Onozato et al., 2017 N/A Human, male: HSC-4-Fucci Onozato et al., 2017 N/A Human, female: SQUU-A (oral squamous cell carcinoma) Morifuji et al., 2000 N/A Human, female: SAS-Fucci stably expressing KRTAP2-3 This paper N/A Human, female: SAS-Fucci KRTAP2-3 KO This paper N/A Human, female: SAS-Fucci stably expressing ZBED2 This paper N/A Human, female: SAS-Fucci stably expressing ENC1 This paper N/A Human, female: SAS-Fucci ENC1 KO This paper N/A Human, female: SAS-Fucci Slug KO This paper N/A Human, female: 293FT (embryonal human kidney), Thermo Fisher Scientific R70007; RRID: CVCL_6911 Experimental models: Organisms/strains Mouse: BALB/cSlc-nu/nu Japan SLC N/A Oligonucleotides primers to generate the pENTR201KRTAP2-3-HA vector; see Table S4A This paper N/A (Continued on next page) Cell Reports 40, 111411, September 27, 2022 e1

    Article Title: Transforming growth factor-β1 induces matrix metalloproteinase-9 expression in rat vascular smooth muscle cells via ROS-dependent ERK-NF-κB pathways.
    Article Snippet: Both matrix metalloproteinase-9 (MMP9) and transforming growth factors-b1 (TGF-b1) are the important factors in the pathogenesis of the aortic aneurysm (AA) and aortic dissection (AD).. Recent studies have shown that inhibition of reactive oxygen species (ROS) production, extracellular signal–regulated kinase 1/2(ERK1/2) or NF-jB pathways is able to suppress aneurysm formation.. The median layers of arterial walls are mainly the vascular smooth muscle cells (VSMCs), while the pathogenesis of AA and AD is closely related to the changes in the median layer structure.



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